Michael Bartlett, Ph.D. Professor & Graduate Coordinator Pharmaceutical and Biomedical Sciences Office: Room 376, R.C. Wilson Pharmacy Phone: (706) 542-5390 E-mail: bartlett@rx.uga.edu

Biosketch

B.S. Chemistry	North Carolina State University	Raleigh, NC	1990
Ph.D. Analytical Chemistry	Georgia Institute of Technology	Atlanta, GA	1994
Postdoctoral Associate	University of Utah	Salt Lake City, UT	1994-95
Assistant Professor	University of Georgia	Athens, GA	1995-2001
Associate Professor	University of Georgia	Athens, GA	2001-Present

Honors and Awards
2005 G.E. Philbrook Award for Undergraduate Teaching, Northeast Georgia Chapter of the American Chemical Society
2002 Named College of Pharmacy Teacher of the Year
2000 & 2001 Named Phi Delta Chi, College of Pharmacy Teacher of the Year
2000 American Association of Colleges of Pharmacy New Investigator Award
1999 American Society for Mass Spectrometry Young Investigator Award
1994 Outstanding Dissertation Award, Georgia Institute of Technology
1990 Award from International Society for the Study of Xenobiotics for Significant

Research Interests
Research in our laboratory centers on applications of analytical chemistry to the study of biological problems including: (a) studies of the absorption, distribution, metabolism and excretion (ADME) of drug substances and environmental toxicants; and (b) the separation of drug substances using capillary electrophoresis.

A. Our studies on the ADME of drug substances and environmental toxicants with a goal of developing methods for assessing fetal exposure. We have developed an animal model, using pregnant Sprague-Dawley rats, which has allowed us to study the placental transport of various drug substances.

We are now using our expertise in LC-MS/MS and the pregnant rat model to study the maternal-fetal transport of anti-viral compounds. The ultimate goal is to improve treatments for preventing vertical transmission of HIV. Presently, AZT is the only drug, which has been shown clinically to be effective in preventing maternal-fetal transmission of HIV. We have been studying azidodeoxyuridine (AZDU), acyclovir, lamivudine, abacavir, zalcitabine, didanosine and stavudine to see how these compounds relate to AZT for fetal uptake. One of the most interesting aspects of this work has been the investigation of pharmacokinetic drug-drug interactions among combination therapies. This work is being conducted in collaboration with Dr. Catherine A. White in the College of Pharmacy.

We are also involved in studies of the common environmental toxicant trichloroethylene (TCE) and its metabolites. To date we have concentrated on the development of sensitive GC-MS and LC-MS-MS methods to assay these compounds. We have completed our development for the analysis of TCE and are using this method to investigate tissue distributions and trace-level pharmacokinetics of this compound. We are continuing our efforts to determine the metabolites and are exploring new techniques such as solid phase microextraction to improve on our existing method for the determination of TCE. These studies would allow for realistic risk assessment of this common environmental contaminant. This work has been done in collaboration with Dr. James V. Bruckner in the College of Pharmacy.

B. Recently there has been an increase in the number of biotechnology products entering the health care system. These molecules are proteins, oligonucleotides, oligosaccharides or combinations of these classes of biomolecules. These molecules are quite different from traditional small organic molecule pharmaceutical agents. These differences have created a significant challenge for the bioanalytical chemist to generate the preclinical and clinical data to support the development of these new agents. Many of the methods used for quantitation of small organic molecules are not easily adaptable to larger biomolecules. Therefore, industry turned to traditional molecular biology techniques such as immunoassays, quantitative PCR and hybridization for quantitative results. However, these assays also have limitations related to their precision, specificity and time. Therefore, there is an urgent need to find improved bioanalytical methods.

Our work with the quantitation of macromolecules has been quite exciting. We have successfully applied electrospray ionization using selected ion monitoring to study a series of polypeptides with molecular weights between 3000-4000 Daltons. We are currently expanding our efforts from polypeptides to therapeutic oligonucleotides using electrospray ionization time of flight mass spectrometry and capillary gel electrophoresis with laser induced fluorescence detection.

Representative Publications
G. Zhang, A.V. Terry and M.G. Bartlett, Liquid Chromatography / Tandem Mass Spectrometry for the Simultaneous Determination of Olanzipine, Risperidone, 9-Hydroxyrisperidone, Clozapine, Haloperidol and Ziprasidone in Rat Plasma, Rapid Communications in Mass Spectrometry, 2007, 21, 920-928.

S.R. Lewis, C.A White and M.G. Bartlett, Simultaneous Determination of Abacavir and Zidovudine From Rat Tissues Using HPLC With Ultraviolet Detection, Journal of Chromatography B, 2007, 850, 45-52.

L.N. Williamson and M.G. Bartlett, “Quantitative Liquid Chromatography-Time of Flight Mass Spectrometry.” Biomedical Chromatography, 2007, 21, 567-576.

L.N. Williamson, A.V. Terry and M.G. Bartlett, Determination of Chlorpyrifos and its Metabolites in Rat Brain Tissue Using Coupled-Column Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry, Rapid Communications in Mass Spectrometry, 2006, 20, 2689-2695.

D.C. Delinsky, K.T. Hill, C.A. White and M.G. Bartlett, Quantitation of the Polypeptide Galanin by Protein Precipitation and External Calibration LC-MS, Journal of Liquid Chromatography and Related Technologies, 2006, 29, 2341-2351.